This research was aimed to observe the performance of lipase from rhizopus oryzae and aspergillus niger in hydrolyzing sardine oil in order to get high omega-3 glycerides. Hydrolysis were carried out in 1 g of sardine oil 1 ml of phosphate buffer reciprocal waterbath shaker 100 strokes/minute temperature 37 C 200 U for both of lipase for 12 hours. The triglyceride diglyceride monoglyceride and free fatty acid contents as the products of the 12 h hydrolysis with lipase from R. oryzae were 37 21 7 and 35% respectively and 55 21 8 and 16% with lipase from A. niger respectively. Both lipases made 40% increasingly in the EPA content of triglyceride. Hydrolysis of sardine oil by lipase from R. oryzae increased 70% of DHA in the triglyceride and 100% in the mizture of mono- and diglyceride. By lipase from A. niger DHA in the triglyceride and in the mixture of mono and diglyceride increased 50 and 25% respectively.