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  <title>Dideoxy-sanger, suatu metoda penentuan urutan nukleotida DNA</title>
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  <namePart>Azhar, Minda</namePart>
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  <publisher>p Eksata: berkala ilmiah bidang MIPA (Univ. Neg. Padang)</publisher>
  <dateIssued></dateIssued>
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  <languageTerm type="text">Indonesia</languageTerm>
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  <extent>Sumber artikel:Jurnal. Halaman: p. 68-80</extent>
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 <note>Sanger dideoxy sequencing method utilizes base-specific termination during in vitro synthesis of DNA. The basis for terminating the elongation of the DNA chain is the use a nucleoside triphosphate containing a ribose sugar reduced in both the 2  and 3   positions (ddNTP). When four normal dNTPs (one of them is labeled at its a position with 32P or 35 S)  are mixed with the respective ddNTP in four different reaction tubes  chain termination that ended in nuclitide A C G and T will occur. When products are analyzed  radioactive signals will be achieved for all bands on the ladder gel.</note>
 <subject authority="">
  <topic>NUKLEOTIDA</topic>
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 <classification>500.7 EKS</classification>
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  <physicalLocation>UPT Perpustakaan UM Koleksi Bahan Pustaka Perpustakaan UM</physicalLocation>
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