This is a study on carotenoid from extracted from Minyak Buah Merah (Pandanus conoideus) in therapy simulation usinghigh dose of Beta-carotene on two lymphocytes-that of a healthy subject and a leukemia patient. Superoxide production in miyhocondrial lymphocyte as a result of Beta-carotene exposure was examnied using a flow cytometer. The test finds that superoxide (a specific mitochondrial free radical) increases easier and faster in leukemic lymphocytes than in normal lymphocytes. The result of the study support previous meta-analysis studies that high dosages of Beta-carotene and vitamin E suplements as antioxidant should be discouraged because it increases all-cause mortality.