The aim of this study was to observe tissue respon of rat that was exposed to chronic systemic hypoxia by analyzing the oxidative stress in liver tissue. twenty male Sprague-Dawley rats were induced by chronic systemic hypoxia by kept them in hypoxid scamber (10% O2 90% N2) for 1 3 7 and 14 day(s). All rats were sacrificed with ether anesthesia after hypoxia treatment. Liver tissues were analyzed using parameters of oxidative stress malondialdehyde (MDA) with tBARS test and endogenous antioxidant gluthatione reduced form (GSH). The study showed that chronic systemic hypoxia induction caused oxidative stress in liver tissues was increased significantly on day-1 day-3 day-7 and day-14 compared to control group (ANOVA LSD p lt 0.05). The differences between day-3 day-7 and day-14 was not significant. In contrast liver GSH content was progressively decreased significantly since day-1 of hypoxia until the end of experiment (ANOVA LSD p lt 0.05). Statistical analysis revealed that there is a strong correlation between MDA and GSH concentration in liver tissues (Pearson - 0.993). It was concluded that oxidative stress present in liver tissue of rat induced by chronic systemic hypoxia.