Chemistry courses in higher education have traditionally been composed of lectures problem-solving sessions and laboratories. This study which took place at the Technion-Israel Institute of Technology was aimed at developing a freshmen Web-based chemistry course and investigating the performance of the students who use it. The research population consisted of seven chemistry faculty and six chemistry teaching assitants. Research tool included semi-structured personal interviews with faculty teaching assistants and experimental students a students survey and pre- and post-test.