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  <title>Comparative Analysis of Caffeine Content in Cold and Hot Brewed Robusta Coffee Using High-Performance Liquid Chromatography (HPLC)</title>
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  <namePart>Irma Rahmawati; Shofian Habib Asrori</namePart>
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  <publisher>Serial JKPK (Jurnal Kimia dan Pendidikan Kimia)&#13;
UNS</publisher>
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  <languageTerm type="code">ind</languageTerm>
  <languageTerm type="text">Indonesia</languageTerm>
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  <extent>Sumber artikel:Jurnal. Halaman: 15-25</extent>
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 <note>Abstract  Coffee is one of the most popular beverages globally  cherished for its unique taste  aroma  and the stimulating effects of its caffeine content. The proliferation of creative coffee shops has introduced various new methods for enjoying coffee  including cold and hot brew techniques. These processing techniques can significantly influence the physicochemical characteristics of coffee  particularly its caffeine content. This study compares the caffeine content in Robusta coffee using cold and hot brewing techniques. The hot brew coffee was prepared using water at approximately 96 deg C with a French press for six minutes. In contrast  the cold brew method involved brewing with water at room temperature (20-25 deg C) using a French press  followed by storage for 12 hours in a refrigerator (2-8 deg C). Qualitative analysis involved examining the color reaction  while quantitative analysis was conducted using High-Performance Liquid Chromatography (HPLC). HPLC is a highly accurate method that is extensively used in the food and pharmaceutical industries. The results indicated that the caffeine content in cold-brewed Robusta coffee was significantly higher  with a concentration of 44.63  mu g/mL  plusmn  0.199% and a Relative Standard Deviation (RSD) of 0.4459%. Conversely  hot-brewed coffee showed a caffeine concentration of 23.96  mu g/mL  plusmn  0.278%  with an RSD of 1.1602%. The parametric Analysis of Variance (ANOVA) revealed a significance value of 0.000 (p  lt  0.05)  indicating a significant difference in caffeine levels between hot-brewed and cold-brewed coffee. These findings suggest that the choice of Robusta coffee processing technique can be crucial for individuals with specific health conditions seeking to manage their caffeine intake.      Keywords  Caffeine  Cold Brew  Hot Brew  HPLC  Robusta Coffee       </note>
 <subject authority="">
  <topic>1. KOPI - KADAR KAFEIN</topic>
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 <classification>540 JKP</classification>
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  <physicalLocation>UPT Perpustakaan UM Koleksi Bahan Pustaka Perpustakaan UM</physicalLocation>
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